High Resolution Mass Spectrometry

Team head:  Jerzy Silberring, Ph.D.   Professor   Staff: 6

The group joins scientists of the Regional Laboratory and the Faculty of Chemistry, Jagiellonian University.

Research topics:

• identification of proteome in drug dependence
• discovery of novel metabolic pathways for neuropeptides (primarily opioid peptides)
• role of proteinases in patophysiology of drug addiction and pain
• role of catecholamines in the immune system

Research equipment:

• Doubly-focusing mass spectrometer Finnigan MAT 95 with EI and electrospray sources.  
• MALDI TOF instrument Bruker Reflex IV with PSD option.  
• GC/MS "Magnum" with EI/CI ionization.  

International collaboration:

• University of Goeteborg, Department of Clinical Neurosciences, Section of Psychiatry and Neurochemistry, S-431 80 Uppsala, Sweden;
• University of Uppsala, Department of Pharmaceutical Biosciences, S-751 24 Uppsala, Sweden;
• Tohoku College of Pharmacy, Department of Pharmacology, Sendai 981, Japan;
• Technical University Berlin, Institute of Organic Chemistry, Berlin, Germany.

Contact:

Selected publications:

• Suder P., Sciubisz A., Smoluch M. and Silberring J., Neuropeptide processing. In: Mass Spectrometry and Hyphenated Techniques in Neuropeptide Research. (J.Silberring and R. Ekman eds), Wiley & Sons, New York (2002, in press).
• Bergquist J., Sciubisz A., Kaczor A. and Silberring J., Catecholamines and methods for their identification and quantitation in biological tissues and fluids. J. Neurosci. Meth. 113, 2002, 1-13.
• Suder P., Wade D., Legowska A., Kotlinska J., Rolka K., Silberring J., Dynorphin A inhibits nociceptin-converting enzyme from the rat spinal cord, Biochem Biophys Res Commun, 287(4), 2001, 927-31.
• Kotlinska J., Suder P., Legowska A., Rolka K. and Silberring J., Orphanin FQ/nociceptin inhibits morphine withdrawal, Life Sci., 66, 2000, 119-123.
• Banbula A., Mak P., Bugno M., Silberring J., Dubin. A., Nelson D., Travis J., Potempa J., Prolyl tripeptidylpeptidase from Porphyromonas gingivalis: A novel enzyme with possible pathological implications for the development of periodontitis, J. Biol. Chem., 274, 1999, 9246-9252.

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